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Experimental procedures yeast twohybrid screeningthe yeast twohybrid system match maker ii clontech was used. The journal of biological chemistry 20170120 the journal of biological chemistry follow new on 2017 jan 20. The ability of trolox 6hydroxy2,5,7,8tetramethylchromane2carboxylic acid, a watersoluble vitamin e analogue, to prevent oxidative damages is well characterized, but the mechanisms. The crude deprotected oligonucleotides were purified by size exclusion chromatography on a g25 sephadex amersham pharmacia biotech column using distilled, deionized water as the eluent.
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